Hepatocellular carcinoma (HCC) is a the most common type of liver cancer and the third leading cause of cancer deaths in the world. Cirrhosis of the liver is a major risk and contributing factor for HCC. The goal of project one is to identify the genes that are associated with regressing tumors (regardless of type of treatment) vs. those that are growing from the C3HeB/FeJ mouse model. Normal liver is used as a control. The results will be useful in identifying new therapeutic targets and potential drug combinations which could lead to more efficient treatments. Project two is related to knockdown of a specific protein that results in HCC in a different mouse model. The goal in the second project is to identify the genes and pathways that correlate with this protein.
Sex/Age | Viral Infection | Tumor Differentiation | No. of Tumors | Vascular Invasion | TNM* Stage |
M/62 | HBV(-)HCV(-) | Well | 1 | No | II |
F/29 | HBV(+)HCV(-) | Well | 1 | No | II |
M/56 | HBV(+)HCV(-) | Moderately | No | II | |
M/55 | HBV(+)HCV(-) | Moderately | 1 | No | II |
F/39 | HBV(+)HCV(-) | Moderately | 1 | No | II |
M/44 | HBV(+)HCV(-) | Moderately | 1 | Yes | III |
M/47 | HBV(+)HCV(-) | Moderately | 1 | No | II |
M/48 | HBV(+)HCV(-) | Poorly | 1 | Yes | III |
Drs. Christi Walter and Lily Dong from the Department of Structural and Cellular Biology at UT Health Science Center in San Antonio.
Jessica Zavadil, Maryanne Herzig, Kim Hildreth, Amir Foroushani, William Boswell, Ronald Walter, Robert Reddick, Hugh White, Habil Zare. “C3HeB/FeJ Mice mimic many aspects of gene expression and pathobiological features of human hepatocellular carcinoma.” Molecular carcinogenesis 58.3 (2019): 309-320.
Protemoe and the APEX1 interactome: Data-independent analysis Mass spectrometry (DIA-MS) done on 3 biological replicates of 2 HCC cell lines (SNU398 and Huh7) and an immortalized hepatocyte line (THLE2). Also, we have 1 biological replicate of a primary hepatocyte organoid derived from a patient (UTHSS-28T).
Our goal is to understand if the APE1 interactome is 1) different in HCC cell lines vs non tumor cells, 2) different between two HCC cell lines with overexpressed APEX1 (SNU398 vs Huh7), 3) and how it compares to that described in Ayyildiz 2020. Christi sent these data to Habil on 2020-12-02 in an email entitled: “M2021-026 Scaffold DIA and Excel files”.