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====== Gene networks inference ======
===== Objectives =====
Biological processes in a cell often require intricate coordination between //multiple// genes and proteins. The goal of this project is to infer useful biological and clinical information from large networks of thousands of genes. We develop an integrative approach to analyze co-expression and DNA methylation patterns in a single model. The results will be useful in pinpointing the cause and mechanism of complex diseases such as cancer. Our findings can potentially open doors to new targets for novel treatment plans. \\
{{:wiki:public:integration2.png?direct&800|}}
\\
===== Collaborators =====

[[http://www.bcgsc.ca/faculty/dr-aly-karsan-md/the-karsan-lab/karsan-lab-page|Dr. Aly Karsan]] from British Columbia Cancer Agency.\\

===== Papers =====

- A. Zainulabadeen et al., Underexpression of Specific Interferon Genes Is Associated with Poor Prognosis of Melanoma, [[http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0170025|PLoS One]] 2017, 12(1). \\  "Using our recently developed gene network model, we identified biological signatures that confidently predict the prognosis of melanoma. We showed that our predictive model assesses the risk more accurately than the traditional Clark staging method."
  - Foroushani, Amir, et al. "Large-scale gene network analysis reveals the significance of extracellular matrix pathway and homeobox genes in acute myeloid leukemia: an introduction to the Pigengene package and its applications." [[https://bmcmedgenomics.biomedcentral.com/articles/10.1186/s12920-017-0253-6|BMC medical genomics]] 10.1 (2017): 16.
  - Agrahari, Rupesh, et al. "Applications of Bayesian network models in predicting types of hematological malignancies." [[https://www.nature.com/articles/s41598-018-24758-5|Scientific Reports]] 8.1 (2018): 6951.

===== Software =====

**Pigengene:** This R package provides an efficient way to perform network analysis and to infer biological signatures from gene expression profiles. The signatures are independent from the underlying platform, e.g., it can infer the signatures using data from microarray and evaluate them in an independent RNA Seq dataset. It is approved by, and publicly available from, [[https://bioconductor.org/packages/Pigengene|Bioconductor]].

**iNETgrate:** [[https://bitbucket.org/habilzare/genetwork/src/master/code/iNETgrate/|This]] R package is useful to integrate DNA methylation and gene expression data into //a single //network. This approach leads to identification of more robust gene modules compared to conventional coexpression networks. The package will be publicly available after review and approval by Bioconductor.

===== Data =====

- mRNA expression and Mutation data from [[:novartis_data|Novartis]]. \\  Broad-Novartis Cancer Cell Line Encyclopedia ([[http://www.broadinstitute.org/ccle/home|CCLE]], [[http://www.nature.com/nature/journal/v483/n7391/full/nature11003.html|Barretina]] et al.).About 500 cell lines from different human cancers. The goal here is to predict drug responses in particular in synergistic settings. Specifically, BROWS > DATA > \\  a. mRNA expression > gene-centric RMA-normalized mRNA expression data > the gctx [[http://www.broadinstitute.org/ccle/data/browseData?conversationPropagation=begin|file]]. ([[:gene_networks_inference|How to]] read a gctx file?) \\  b. Pharmacological profiling Drug data > Pharmacologic profiles for 24 anticancer drugs across 504 CCLE lines. \\  c. [[:file_view_ccle_chris_clustering.csv_535042602_ccle_chris_clustering.csv|Clustering]] and gene [[:file_view_ccle_chris_go.csv_535042614_ccle_chris_go.csv|ontology]] analysis done by Dr. Chris [[https://www.linkedin.com/profile/view?id=145098421&authType=NAME_SEARCH&authToken=xR3c&locale=en_US&srchid=1029939271418481101510&srchindex=1&srchtotal=1&trk=vsrp_people_res_name&trkInfo=VSRPsearchId:1029939271418481101510,VSRPtargetId:145098421,VSRPcmpt:primary|Gaiteri]].
  - RNA-seq from about a hundred [[:alys_data|AML]] and MDS cases are available from Karsan lab. The goal here is to identify the general underling mechanisms of the disease, and to compare them with the relapse factors. More specific questions are a) What pathways are different in AML than MDS? b) Are there pathways which can define AML subtypes, which are expected to exist due to differences in prognosis? c) What are the molecular mechanisms of [[http://dsas9a9gxtv2e.cloudfront.net/content/haematol/95/10/1623/F1.large.jpg|transformation]] of some MDS cases to AML?
  - "[[http://www.ncbi.nlm.nih.gov/geo/|GEO]], a public functional genomics data repository" ([[https://www.biostars.org/p/97370/|source]]). Includes over 4,000 leukemia subjects in the MILES series, a particular microArray study that contains around 400 AML and 300 MDS cases.
  - NCI-60 cell lines ([[http://www.cbioportal.org/public-portal/study.do?cancer_study_id=cellline_nci60|cBio]] portal, [[http://dtp.nci.nih.gov/branches/btb/ivclsp.html|DTB]]).
  - Genentech data set [[http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.3080.html|published]] in 2014 (Klinj et al.), RNA-seq for 675 cell lines including 15 AMLs, and response to 5 drugs.
  - Sanger data set [[http://www.nature.com/nature/journal/v483/n7391/full/nature11005.html|published]] in 2012 (Garnet et al.), similar to CCLE data with 639 cell lines and 130 compounds.
  - GlaxoSmithKline data set [[http://cancerres.aacrjournals.org/content/70/9/3677.long|published]] in 2010 (Greshock et al.), similar to CCLE data with 311 cell lines and 19 compounds.
  - Nucleic Acids Research online Molecular Biology Database [[http://nar.oxfordjournals.org/content/41/D1/D1.abstract?ijkey=a782763acb573716f2620e420a35a6d3fbaa3cf5&keytype2=tf_ipsecsha|Collection]]. \\  lists 1512 miscellaneous online databases.
  - RNA-seq data of over 100 Xiphophorus fish treated with light under different conditions such as dosage and wavelength. 20-30 controls are also available from Walter [[http://mbrg.chemistry.txstate.edu/|Lab]] [[:https:docs.google.com_spreadsheets_d_1og2dooykcfcidycipgo6q50nwtumflu-ll8-irkr_6u_edit#gid_0|table]]].
  - [[http://www.reuters.com/article/2015/09/22/us-astrazeneca-cancer-idUSKCN0RM0MG20150922|AstraZeneca's]] crowd sourcing initiative as part of the DREAM Challenge. ~10,000 tested combinations measuring the ability of drugs to destroy cancer cell lines, and the corresponding genomic information.
  - Breast Cancer datasets: We will examine the generalizability of the method that we developed for haematological malignancies (AML/MDS) by examining its performance on several breast cancer datasets: 209 ER samples from Wang et al's [[http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=gse2034|dataset ]](GEO). ([[http://www.nature.com/ncomms/journal/v1/n4/full/ncomms1033.html|Paper]]), 201 ER samples from Miller et al's [[http://www.ncbi.nlm.nih.gov/geo/geo2r/?acc=GSE3494&platform=GPL96|dataset]] (GEO), as well as expression data from [[https://www.ebi.ac.uk/ega/studies/EGAS00000000083|METABRIC]] study ( ~2000 samples, hosted by EGA) ([[http://www.nature.com/nature/journal/v486/n7403/full/nature10983.html|paper]]).
  - Microarray expression profiles of 1005 colorectal cancer patients from 13 independent cohorts ([[http://www.cancer-systemsbiology.org/Papers/JAMA-2015.pdf|paper]]).
  - Gene expression [[https://docs.google.com/spreadsheets/d/1oG2doOyKcfCidYciPgO6Q50nwtUmflU-Ll8-iRkr_6U/edit#gid=0|data]] of fish exposed to light (Walter Lab).
  - 16 pairs of tumor-normal samples from fish with [[:file_view_count_table_extra_32_samples_cpm.csv_578966565_count_table_extra_32_samples_cpm.csv|melanoma]] (Walter Lab).
  - 499 prostate adenocarcinoma ([[https://tcga-data.nci.nih.gov/tcga/tcgaCancerDetails.jsp?diseaseType=PRAD&diseaseName=Prostate adenocarcinoma|TCGA]], Provisional) samples. Low risk cases are "Disease Free" for at least 5 years and the "Recurred" ones are high risk. The relevant clinical data are shown in "Disease Free (Months)" and "Disease Free Status" columns in [[http://www.cbioportal.org/study.do?cancer_study_id=prad_tcga#|cBioPortal]], respectively.
  - 470 skin [[https://tcga-data.nci.nih.gov/tcga/tcgaCancerDetails.jsp?diseaseType=SKCM&diseaseName=Skin Cutaneous Melanoma|cutaneous]] melanoma samples from TCGA. The clinical data for survival analysis are shown in "Disease Free (Months)", "Disease Free Status", and "Days to Last Followup" columns.
  - 200 AML cases from TCGA (LAML dataset). Available data types include gene expression , DNA-methylation, CNV, mutation, etc. TCGA data moved to [[https://gdc-portal.nci.nih.gov/|GDC]] but DNA-methylation is not there. Instead, it can be retrieved from GDC Legacy Archive or the original [[https://tcga-data.nci.nih.gov/docs/publications/laml_2012/|paper]].
  - German [[https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE37642|AMLCG]] 1999 provides microarray data of 562 AML samples.
  - Papaemmanuil, Elli, et al. "Genomic classification and prognosis in acute myeloid leukemia." [[http://www.nejm.org/doi/full/10.1056/NEJMoa1516192#t=article|NEJM]] 374.23 (2016): 2209-2221. \\  The mutations of 111 genes in over **1,500 AML**  cases are reported. The authors used this information to classify cases into groups and showed these groups have different prognosis. I.,e., [[https://www.mskcc.org/sites/default/files/node/2246/documents/discrete-cpe.pdf|concordance]] (probability estimates) improves from 64% using only the European LeukemiaNet criteria to 71%. Using the alternative allele frequency, they estimated the time of occurrence for the driver mutations. The data are available through the links in the corresponding [[http://www.nature.com/ng/journal/v49/n3/full/ng.3756.html|Nature]] paper [[[:ng.3756.pdf?media=ng.3756.pdf|pdf]]]. Information on downloading these data is contained in the readme file found in genetwork:~/proj/genetwork/data/AML/gerstung/readme.txt. In particular, we have access to [[https://www.ebi.ac.uk/ega/studies/EGAS00001000275|EGAS00001000275]] through [[https://ega-archive.org/|EGA]] Archives. See [[:habils_lab_notebook|Habil's]] note on 2017/09/05 for more detail. Any member of Oncinfo Lab who touches (analyzes or views) these data from Sanger Institute must read and abide to the [[:sanger_data_agreement_2017-08-09.pdf?media=sanger_data_agreement_2017-08-09.pdf|agreement]].
  - RNA, DNA methylation, whole genome, etc. data of 960 (pediatric?) AML cases are available from [[https://ocg.cancer.gov/programs/target/acute-myeloid-leukemia|TARGET]] AML study.
  - AML-NK gene expression data (RNA-Seq) from three datasets (TCGA, Leucegene, and PMP/BCCA). [[https://docs.google.com/a/princeton.edu/document/d/1tB75BDAoG6-ggkoKzxF_f8anTnaP0lOAZ4MG-wEWCyk/edit?usp=sharing|Full description]].
  - [[https://docs.google.com/document/d/1Q6tuMDw4fweRQNttmiG3NEZiBoe2JcRj7M1Qg-_rCgk/edit|List]] of available AML datasets with DNA methylation or gene expression data.
  - Genomic Data Commons ([[https://portal.gdc.cancer.gov/repository|GDC]]), which contains TCGA data and more.
  - [[https://amp.pharm.mssm.edu/archs4/|ARCHS4]], which was developed at the Icahn School of Medicine at Mount Sinai, and provides tools to download and analyze RNA-Seq data including single-cell gene expression.

=====   =====

===== Related work =====

- Zhang, B. //et al.//  Integrated systems approach identifies genetic nodes and networks in late-onset Alzheimer's disease. //[[http://www.cell.com/abstract/S0092-8674(13)00387-5|Cell]]//**153**, 707–720 (2013). [{{:zhang2013.pdf|pdf}}  ] \\  Methodology is based on [2] plus they train **Bayesian networks**  to infer causal structure based on RNA-seq. MCMC was used to optimize BIC score, and \\ 1/3 of 1000 network models were averaged.[[http://www.alzforum.org/webinars/can-network-analysis-identify-pathological-pathways-alzheimers|This]] webinar is a high level description of the methodology.
  - Emilsson, V. //et al.//  Genetics of gene expression and its effect on disease.//[[http://www.nature.com/nature/journal/v452/n7186/full/nature06758.html|Nature]]//**452**, 423–428 (2008). [[:http:file_view_emilsson2008.pdf_521380262_emilsson2008.pdf|pdf]], {{:emilsson2008-supp.pdf|Supp}}  ] (based on their references [29-31], uses [[http://www.sciencemag.org/content/297/5586/1551.full.pdf|this]] topological overlap measure)
  - Identifying Gene Regulatory Networks from Gene Expression Data, [[http://web.cs.ucdavis.edu/~filkov/papers/chapter.pdf|Handbook]] of Computational Molecular Biology (2005) [{{:filkov_chapter27.pdf|pdf}}  ]. \\  A good but old book chapter.
  - [[http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0020130|Integrating Genetic and Network Analysis to Characterize Genes Related to Mouse Weight]] (Identified modules in networks, "A pair of genes is said to have high topological overlap if they are both strongly connected to the same group of genes.", [[http://labs.genetics.ucla.edu/horvath/htdocs/CoexpressionNetwork/MouseWeight/|Software]])
  - Gene correlation network analysis [Wikipedia [[http://en.wikipedia.org/wiki/Weighted_correlation_network_analysis|page]]]
  - Friedman, Nir, et al. "Using Bayesian networks to analyze expression data." Journal of computational biology 7.3-4 (2000): 601-620.[{{:friedman2000.pdf|pdf}}  ] \\  A relatively old but highly cited, (~2700) original paper.
  - Ruan, Jianhua, Angela K. Dean, and Weixiong Zhang. "A general co-expression network-based approach to gene expression analysis: comparison and applications." //[[http://www.biomedcentral.com/1752-0509/4/8|BMC]] systems biology//4.1 (2010): 8. (Dr. Jianhua Ruan from San Antonino)
  - Nagrecha, Saurabh, Pawan J. Lingras, and Nitesh V. Chawla. "Comparison of gene co-expression networks and bayesian networks." //Intelligent Information and Database [[https://www3.nd.edu/~dial/papers/ACIIDS2013.pdf|Systems]]// . Springer Berlin Heidelberg, 2013. 507-516. \\ Simple description and some relatively old literature review. \\ "Bayesian networks emerge as a more informative tool to determine the causal structure."
  - Systems Biology: The inference of networks from high dimensional genomics data, lecture by Yeung 2011 [{{:yeun2011-systemsbiology.ppt|ppt}}  ] (a good introduction to application of Bayesian networks in co-expression network)
  - Hong, Shengjun, et al. "Canonical correlation analysis for RNA-seq co-expression networks." //Nucleic acids [[http://www.ncbi.nlm.nih.gov/pubmed/23460206|research]]//41.8 (2013): e95-e95. (improved co-expression analysis for RNA-seq)
  - Li, Bingshan, et al. "[[http://www.nature.com/jid/journal/v134/n7/full/jid201428a.html|Transcriptome Analysis of Psoriasis in a Large Case–Control Sample: RNA-Seq Provides Insights into Disease Mechanisms.]]" //Journal of Investigative Dermatology//  (2014). (used co-expression network analysis on RNA-seq from 42 samples to study gene regulatory circuits in psoriasis)
  - [[http://web.stanford.edu/group/wonglab/doc/RNA-seq-talk-JSM2010.pdf|Analysis]] of RNA‐Seq Data, an introduction by Wong, 2010.
  - Ellis, Byron, and Wing Hung Wong. "Learning **causal**  Bayesian network structures from experimental [[http://web.stanford.edu/group/wonglab/doc/EllisWong-061025.pdf|data]]." //Journal of the American Statistical Association//  103.482 (2008): 778-789.
  - Barretina et al. "The Cancer Cell Line Encyclopedia enables predictive modeling of anticancer drug sensitivity." [[http://www.nature.com/nature/journal/v483/n7391/full/nature11003.html|Nature]] 483.7391 (2012): 603-607 [{{:barretina2012.pdf|pdf}}  ]. \\  It provides CCLE, a valuable dataset produced by **Novartis**; mRNA expression data, responses of 24 compounds, and targeted sequencing on ~500 cell lines.
  - Friedman, Nir, et al. "Using Bayesian networks to analyze expression data."//Journal of computational [[http://www.cs.huji.ac.il/~nir/Papers/FLNP1Full.pdf|biology]]//7.3-4 (2000): 601-620. \\ Has a good introduction to Bayesian networks and learning causal patterns for beginners.
  - Al-Lazikani, Bissan, Udai Banerji, and Paul Workman. "Combinatorial drug therapy for cancer in the post-genomic era." //[[http://www.nature.com/nbt/journal/v30/n7/full/nbt.2284.html|Nature]] biotechnology//30.7 (2012): 679-692. \\ "Combinatorial targeted therapy", a good **survey**  including computational methods, successful stories like "identification of synergies between MET and EGFR inhibitors…"
  - Molinelli, Evan J., et al. "Perturbation Biology: inferring signaling networks in cellular systems." //[[http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003290|PLoS]] computational biology//9.12 (2013): e1003290. \\ "…inferring network models that predict the response of cells to perturbations which may be useful in the design of combinatorial therapy against cancer. Claims differential equations work better than Bayesian networks (Biolearn).
  - Marbach, Daniel, et al. "Revealing strengths and weaknesses of methods for gene network inference." [[http://www.pnas.org/content/107/14/6286.full|PNAS]] 107.14 (2010): 6286-6291. \\  Survey on DREAM results, includes Bayesian network methods.
  - Molinelli, Evan J., et al. "Perturbation Biology: inferring signaling networks in cellular systems." [[http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003290|PLoS]] computational biology 9.12 (2013): e1003290. \\  Belief Propagation technique developed in Sloan Kattering, claimed to be better than BN.
  - The NCI-DREAM Drug Sensitivity Prediction [[http://www.the-dream-project.org/challenges/nci-dream-drug-sensitivity-prediction-challenge|Challenge]] (2012) is a similar project in a smaller scale (2014 Nature [[http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt.2877.html|paper]]). NIEHS-NCATS-UNC DREAM Toxicogenetics [[https://www.synapse.org/#!Wiki:syn1761567/ENTITY|Challenge]] (2013) uses genomic and RNA-seq data from hundreds of cases with slightly different goal.
  - Zhao, J., X. S. Zhang, and S. Zhang. "Predicting cooperative drug effects through the quantitative cellular profiling of response to individual drugs." [[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3944117/pdf/psp201379a.pdf|CPT]]: pharmacometrics & systems pharmacology 3.2 (2014): e102. \\  "By exploring the differential expression profiles, our correlation-based strategy can reveal the synergistic effects of drug combinations." A recent paper which used a simple method and includes a good literature review. A good reference to datasets similar to CCLE.
  - Du, W., and O. Elemento. "Cancer systems biology: embracing complexity to develop better anticancer therapeutic strategies." [[http://www.nature.com/onc/journal/vaop/ncurrent/full/onc2014291a.html|Oncogene]] (2014). \\  A recent review paper explaining the motivation and need for computational methods.
  - Han, Bing, and Xue-wen Chen. "bNEAT: a Bayesian network method for detecting epistatic interactions in genome-wide association studies." BMC [[http://www.biomedcentral.com/1471-2164/12/S2/S9|genomics]] 12.Suppl 2 (2011): S9. \\  Potential collaboration with [[http://www.cs.wayne.edu/xwchen/|Chen]]. Argues that Branch-and-Bound is better than MCMC. Good introduction to BN structure learning.
  - Bayesian Network Learning and Applications in Bioinformatics (2012), \\ Xiaotong Lin's PhD [[http://kuscholarworks.ku.edu/bitstream/handle/1808/10150/Lin_ku_0099D_12362_DATA_1.pdf?sequence=1&isAllowed=y|thesis]] with a good introduction to the basic concepts and challenges.
  - Bansal, Mukesh, et al. "How to infer gene networks from expression profiles."//Molecular systems biology//  3.1 (2007). \\ A relatively old survey which supports Banjo.
  - Mourad, Raphaël, and Christine Sinoquet, eds. Probabilistic Graphical Models for Genetics, Genomics and Postgenomics. [[http://books.google.com/books?hl=en&lr=&id=2URhBAAAQBAJ&oi=fnd&pg=PP1&dq=Probabilistic Graphical Models for Genetics, Genomics, and Postgenomics&ots=8Yweyc5bKz&sig=xhaDE9f-JXHNckj-lgwvSrgSl3g#v=onepage&q=Probabilistic Graphical Models for Genetics, Genomics, and Postgenomics&f=false|Oxford]] University Press, 2014. \\  An excellent recent, relevant book ( e.g. pages 24,123,154,223).P155: For gene networks, maximum number of parents is commonly set to 3.
  - de la Fuente, Alberto. "Gene Network Inference.", [[http://www.springer.com/new & forthcoming titles (default)/book/978-3-642-45160-7|Springer]], 2013 [{{:fuente2013.pdf|pdf}}  ]. \\  Figure 1 explains why a network-based approach can be superior to black box machine learning techniques. Literature review on advantages of system approaches over studying single genes. Limited the maximum number of parents per gene to 5. [[http://www.mountsinai.org/profiles/jun-zhu|Zhu]] et al. explains [[http://icahn.mssm.edu/departments-and-institutes/genomics/about/software/rimbanet|RIMBANet]] developed by himself. "Bayesian networks offer the best performance."
  - Bayesian Networks with R (bnlearn) and Hadoop, 2014, a good [[http://www.slideshare.net/ofermend/bayesian-networks-with-r-and-hadoop|talk]] by \\  Ofer Mendelevitch with introduction to BNs. Discusses large networks too.
  - Network Analysis Workshop, Systems Biology Analysis Methods for Genomic, 2013, [[http://labs.genetics.ucla.edu/horvath/CoexpressionNetwork/WORKSHOP/2013/|UCLA]]. Talk in Bayesian networks by Jun Zhu.
  - Vignes, Matthieu, et al. "Gene regulatory network reconstruction using bayesian networks, the dantzig selector, the lasso and their meta-analysis." PloS [[http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029165#pone-0029165-g008|one]] 6.12 (2011): e29165. \\  A meta-analysis to combine these inference methods by computing a consensus ranking scheme, ranked 1st among 16 in a DREAM challenge, but its superiority was not confirmed in [[http://link.springer.com/chapter/10.1007/978-3-642-45161-4_2|Allouche]] 2014. Used greedy hill-climbing of Banjo.
  - Nagarajan, Radhakrishnan, Marco Scutari, and Sophie Lèbre. Bayesian Networks in R. [[http://link.springer.com/book/10.1007/978-1-4614-6446-4|Springer]], 2013 [{{:bn-r.pdf|pdf}}  ]. \\  A starter-to-advanced book by the author of bnlearn R package, defines a way of comparing networks in foreword section.
  - Schadt, Eric E., et al. "An integrative genomics approach to infer causal associations between gene expression and disease." [[http://www.nature.com/ng/journal/v37/n7/full/ng1589.html|Nature]] genetics 37.7 (2005): 710-717. \\  The original Schadt paper introducing the idea of using genomic data to infer causality in BNs of expression (LCMS).
  - Jiang, Xia, et al. "Learning genetic epistasis using Bayesian network scoring criteria." BMC [[http://www.biomedcentral.com/1471-2105/12/89/|bioinformatics]] 12.1 (2011): 89. \\  On simulated data, Bayesian scoring (BDeu) outperforms minimum description scores such as AIC.
  - Hartemink, Alexander John. Principled computational methods for the validation and discovery of genetic regulatory networks. Diss. [[http://dspace.mit.edu/handle/1721.1/8699#files-area|MIT]], 2001. \\  Hartemink's (author of Banjo) thesis, well explains BDe score and Dirichlet priors.
  - Yu, Jing, et al. "Advances to Bayesian network inference for generating causal networks from observational biological data." [[http://bioinformatics.oxfordjournals.org/content/20/18/3594.full.pdf|Bioinformatics]] 20.18 (2004): 3594-3603. \\  From Hartemink group, ~500 citations. DBN.
  - Yu, Jing, et al. "Using Bayesian network inference algorithms to recover molecular genetic regulatory networks." //3rd International [[http://ftp.cs.duke.edu/~amink/publications/manuscripts/hartemink02.icsb.pdf|Conference]] on Systems Biology//. 2002. \\ From Hartemink group, DBN. Very good explanation and comparison of different scores and search strategies. "With large amounts of data, the BIC is a good approximation to the full posterior (BDe) score and is faster to compute; however, it is known to over-penalize with small amounts of data." "The BDe score works better than the BIC score in recovering genetic regulatory pathways." [Compared to simulated annealing and genetic algorithm,] greedy search is better as it can find the top graph in the least amount of time" (note that their network is small). " 3-category discretization was optimal.
  - Zhu, Jun, et al. "Increasing the power to detect causal associations by combining genotypic and expression data in segregating populations." [[http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.0030069|PLoS]] computational biology 3.4 (2007): e69. \\  The methodology used to learn BNs in Zhang et al. 2013 AD paper.
  - Zhu, J., et al. "An integrative genomics approach to the reconstruction of gene networks in segregating populations." [[http://www.ncbi.nlm.nih.gov/pubmed/15237224|Cytogenetic]] and genome research 105.2-4 (2004): 363-374 [{{:zhu2004.pdf|pdf}}  ]. \\  The methodology used to incorporate genetic data to better learn BNs (e.g. useful in Zhang et al. 2013 AD paper). Edges which appeared in more than 30% of 1000 graphs should be used to make the consensus graph.
  - Steidl, Ulrich G., and Constantine S. Mitsiades. "Therapeutic and diagnostic target gene in acute myeloid leukemia." U.S. [[http://www.google.com/patents/US20140187604|Patent]] Application 14/113,405. \\  Useful for sanity check to see if the genes we identify are known to be important.
  - Kommadath, Arun, et al. "Gene co-expression network analysis identifies porcine genes associated with variation in Salmonella shedding." //BMC [[http://www.biomedcentral.com/1471-2164/15/452|genomics]]//15.1 (2014): 452. \\ A recent study that used WGCNA on RNA-seq.
  - Oldham, Michael C., et al. "Functional organization of the transcriptome in human brain." //Nature [[http://www.nature.com/neuro/journal/v11/n11/full/nn.2207.html|neuroscience]]//11.11 (2008): 1271-1282. \\ WGCNA authors used it to provide biological insight. Validated co-expression with experimentally validated interacting human protein pairs from EBI [[:http:labs.genetics.ucla.edu_horvath_coexpressionnetwork_humanbraintranscriptome_oldham_biocomp08_7-15-08.pdf|slides]]].
  - Iancu, Ovidiu D., et al. "Utilizing RNA-Seq data for de novo coexpression network inference." //[[http://bioinformatics.oxfordjournals.org/content/28/12/1592.full|Bioinformatics]]//28.12 (2012): 1592-1597. \\ "The first RNA-Seq data de novo network inference…. Retained the probes with above median connectivity, resulting in 3618 common probes."
  - Miller, Brady G., and John A. Stamatoyannopoulos. "Integrative meta-analysis of differential gene expression in acute myeloid leukemia." //PLoS [[http://www.plosone.org/article/info:doi/10.1371/journal.pone.0009466|One]]//5.3 (2010): e9466. \\ A list of AML associated genes compiled from 25 published studies [full [[:file_view_aml-25-studies-genes.txt_536702720_aml-25-studies-genes.txt|data]]].
  - Klijn, Christiaan, et al. "A comprehensive transcriptional portrait of human cancer cell lines." //[[http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.3080.html|Nature]] biotechnology// (2014). \\ RNA-seq data for 675 cell lines, also a good and recent source for references and similar data such as CCLE and Sanger 2012 data set. "… clustering of RNA-seq–derived gene expression showed that …the lymphoid cells formed a notably distinct cluster" So there should be potential for interpreting by modules.
  - Garnett, Mathew J., et al. "Systematic identification of genomic markers of drug sensitivity in cancer cells." //[[http://www.nature.com.libproxy.txstate.edu/nature/journal/v483/n7391/full/nature11005.html|Nature]]//483.7391 (2012): 570-575. \\ Sanger data set similar to CCLE data with 639 cell lines and 130 compounds.
  - Haibe-Kains, Benjamin, et al. "Inconsistency in large pharmacogenomic studies." //[[http://www.nature.com/nature/journal/v504/n7480/full/nature12831.html|Nature]]// (2013). \\ "The measured drug response data are highly discordant" between CCLE and Sanger data!
  - Soneson, Charlotte, and Mauro Delorenzi. "A comparison of methods for differential expression analysis of RNA-seq data." //[[http://www.biomedcentral.com/1471-2105/14/91|BMC]] bioinformatics//14.1 (2013): 91.
  - Friedman, Nir, and Daphne Koller. "Being Bayesian about network structure. A Bayesian approach to structure discovery in Bayesian networks." Machine [[http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.111.3429&rep=rep1&type=pdf|learning]] 50.1-2 (2003): 95-125. \\  ~ 600 citations. Tries to address the challenge of learning a large network (thousands of genes) with few (hundreds of) samples using a Bayesian approach. Also, learning a total ordering is suggested. Implemented in catNet.
  - Subramanian, Aravind, et al. "Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles." [[http://www.pnas.org/content/102/43/15545.long|PNAS]] 102.43 (2005): 15545-15550. \\  Over 7K citations. Microarray on mRNA. Criticizes DE analysis. Gene sets are given based on prior knowledge and scored based on association with the phenotype.
  - Choi, YounJeong, and Christina Kendziorski. "Statistical methods for gene set co-expression analysis." [[http://bioinformatics.oxfordjournals.org/content/25/21/2780.full|Bioinformatics]] 25.21 (2009): 2780-2786. \\  Criticizes approaches that are based on gene modules. Identifies changes in gene correlations. Uses gene sets in KEGG and GO. Comparison and introduction to well-known gene set enrichment methods till 2009.
  - Ideker, Trey, et al. "Discovering regulatory and signalling circuits in molecular interaction networks."//[[http://bioinformatics.oxfordjournals.org/content/18/suppl_1/S233.abstract?ijkey=929cb32cdbc8664e6f7801b68995e3bb2523a80d&keytype2=tf_ipsecsha|Bioinformatics]]//18.suppl 1 (2002): S233-S240. \\ "Screening a molecular interaction (protein-protein) network to identify active (altered) subnetworks, i.e., connected regions of the network that show significant changes in expression over particular subsets of conditions." Input: expressions pathways data base, output: altered subnetworks of each pathway.
  - Gene Set Enrichment Analysis ([[http://www.broadinstitute.org/gsea/index.jsp|GSEA]]) is a computational method by Broad that determines whether an a priori defined set of genes (e.g. MSigDB) shows statistically significant, concordant differences between two biological states (e.g. phenotypes). \\  Input: expressions pathway data base, output: p-values for each "pathway".
  - Isci, Senol, et al. "Bayesian network prior: network analysis of biological data using external knowledge."//[[http://bioinformatics.oxfordjournals.org/content/30/6/860.long|Bioinformatics]]//30.6 (2014): 860-867. \\ Gene interaction networks are learned from experimental data using Bayesian networks (BNs) while incorporating external knowledge, such as KEGG and NCI, as Bayesian Network Prior (BNP). Includes links to several useful data sets. Build on their former approach for improving pathways by BNs. Used fold change discretized by kmeans as the value of each random variable (gene).Input: expressions, output: "pathways".
  - Nagarajan, Radhakrishnan, et al. "Functional relationships between genes associated with differentiation potential of aged myogenic progenitors." Frontiers in [[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3059939/pdf/fphys-01-00021.pdf|Physiology]] 1 (2010). \\  Proposed a method to identify the threshold on the strength of edges in a consensus BN based on background noise.
  - Niculescu, Radu Stefan, Tom M. Mitchell, and R. Bharat Rao. "Bayesian network learning with parameter constraints." The Journal of Machine Learning [[http://www.jmlr.org/papers/volume7/niculescu06a/niculescu06a.pdf|Research]] 7 (2006): 1357-1383. \\  "Automatically discovering clusters of voxels that can be more accurately learned with shared parameters"
  - Gillis, Jesse, and Paul Pavlidis. "“Guilt by association” is the exception rather than the rule in gene networks." [[http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1002444|PLoS]] computational biology 8.3 (2012): e1002444. \\  Discussed the difficulties of computational network analysis. "…Functional information within gene networks is typically concentrated in only a very few interactions whose properties cannot be reliably related to the rest of the network".
  - Koski, Timo JT, and John Noble. "A review of bayesian networks and structure learning." [[http://www-users.mat.umk.pl/~wniem/SemMgr/BayesNets/Bayesnetsreview.pdf|Mathematica]] Applicanda 40.1 (2012): 51-103. \\  A review from mathematical view point including applications of algebraic geometry to Bayesian networks!
  - Barabási, Albert-László, Natali Gulbahce, and Joseph Loscalzo. "Network medicine: a network-based approach to human disease. ({{:network_medicine_barabasi_1.pdf|pdf}}  )" [[http://www.ncbi.nlm.nih.gov/pubmed/21164525|//Nature Reviews Genetics// ]]12.1 (2011): 56-68.
  - Kustra, Rafal, and Adam Zagdanski. "Incorporating gene ontology in clustering gene expression data." //Computer-Based Medical Systems, 2006. [[http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=1647629&tag=1|CBMS]] 2006. 19th IEEE International Symposium on//. IEEE, 2006.
  - Dotan-Cohen, Dikla, Simon Kasif, and Avraham A. Melkman. "Seeing the forest for the trees: using the gene ontology to restructure hierarchical clustering."[[http://www.ncbi.nlm.nih.gov/pubmed/19497934|//Bioinformatics//]] 25.14 (2009): 1789-1795. \\  Semi-supervised clustering.
  - **[[http://ai.cau.ac.kr/?f=softwares&m=sicago|see the graphic under section two here.]]**Kang, Bo-Yeong, Song Ko, and Dae-Won Kim. "SICAGO: Semi-supervised cluster analysis using semantic distance between gene pairs in Gene Ontology." [[http://www.ncbi.nlm.nih.gov/pubmed/20348546|//Bioinformatics//]] 26.10 (2010): 1384-1385.
  - Mostafavi, Sara, et al. "GeneMANIA: a real-time multiple association network integration algorithm for predicting gene function." [[http://www.genomebiology.com/2008/9/S1/S4|//Genome Biol// ]]9.Suppl 1 (2008): S4. [[http://genemania.org/|Website]] \\  Predicting gene function using prior biological knowledge and expression.
  - Frost, Hildreth R., Zhigang Li, and Jason H. Moore. "Spectral gene set enrichment (SGSE)." //BMC [[http://www.biomedcentral.com/1471-2105/16/70|bioinformatics]]//16.1 (2015): 70. \\ Enrichment analysis on PCA.
  - Soneson, Charlotte, and Mauro Delorenzi. "A comparison of methods for differential expression analysis of RNA-seq data." //BMC [[http://www.biomedcentral.com/1471-2105/14/91|bioinformatics]]//14.1 (2013): 91. \\ Compared 11 methods including DESeq and edgR. Before remove ([[http://www.nature.com/nbt/journal/v32/n9/full/nbt.2931.html|RUV]]), which estimated expression in compassion to controls.
  - Wagner, Günter P., Koryu Kin, and Vincent J. Lynch. "Measurement of mRNA abundance using RNA-seq data: RPKM measure is inconsistent among samples." Theory in [[http://www.ncbi.nlm.nih.gov/pubmed/22872506|Biosciences]] 131.4 (2012): 281-285. \\  Use TPM instead [[:http:www.homolog.us_blogs_blog_2013_11_22_cshl-keynote-talk-lior-pachter|forum]], [[https://groups.google.com/forum/#!topic/rsem-users/W9RQrZIOzA4|conversion]]].
  - Dillies, Marie-Agnès, et al. "A comprehensive evaluation of normalization methods for Illumina high-throughput RNA sequencing data analysis." [[http://bib.oxfordjournals.org/content/early/2012/09/15/bib.bbs046.long|Briefings]] in bioinformatics 14.6 (2013): 671-683. \\  RPKM is bad. Use DESeq or TMM ([[http://www.nature.com.libproxy.uthscsa.edu/nbt/journal/v32/n9/full/nbt.2957.html|used]] to compare with microarray).
  - Horvath, Steve. //Weighted Network Analysis: Applications in Genomics and Systems Biology//. [[http://link.springer.com/book/10.1007/978-1-4419-8819-5|Springer]] Science & Business Media, 2011. \\  An overview on network analysis by the author of WGCNA.
  - Frost, H. Robert, Zhigang Li, and Jason H. Moore. "Principal component gene set enrichment (PCGSE)." //[[http://arxiv.org/pdf/1403.5148v1.pdf|arXiv]] preprint arXiv:1403.5148// (2014). \\ Used PCA. Defined a couple of statistics to test if a gene set is enriched by a PC. Applied on AML. Later, used PCGSE for Spectral gene set enrichment ([[http://www.biomedcentral.com/1471-2105/16/70#B27|SGSE]]).
  - Conway O’Brien, Emma, Steven Prideaux, and Timothy Chevassut. "The epigenetic landscape of acute myeloid leukemia." Advances in [[http://www.hindawi.com/journals/ah/2014/103175/|hematology]] 2014 (2014). \\  Review paper. Talks about H3K27me and other histone modifications in AML. Also see references [[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3422511/|73]] (knocked down ASXL1 in primary hematopoietic cells and measured H3K27me3), [[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3145345/|6]] (review), [[http://www.nature.com/nrc/journal/v12/n9/full/nrc3343.html|8]] (review), and [[http://www.nature.com/ng/journal/v42/n8/full/ng.621.html|76]] (studied the role of EZH2).
  - Yamazaki, Jumpei, et al. "The epigenome of AML stem and progenitor cells." [[http://www.ncbi.nlm.nih.gov/pubmed/23249680|Epigenetics]] 8.1 (2013): 92-104. \\  "Found no major differences in DNA methylation" but "thousands of genes that change H3K4me3 or H3K27me3"
  - Abdel-Wahab, Omar, et al. "ASXL1 mutations promote myeloid transformation through loss of PRC2-mediated gene repression." Cancer [[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3422511/|cell]] 22.2 (2012): 180-193. \\  Includes Chip-seq analysis of H3K27me3 on AML.
  - Halsey, Lewis G., et al. "The fickle **P value**  generates irreproducible results." Nature [[http://www.nature.com/nmeth/journal/v12/n3/full/nmeth.3288.html|methods]] 12.3 (2015): 179-185. \\  A reference for justifying the need for network analysis.
  - Wang, Bo, et al. "Similarity network fusion for **aggregating data types**  on a genomic scale."//Nature [[http://www.ncbi.nlm.nih.gov/pubmed/24464287|methods]]//11.3 (2014): 333-337. \\ Combined mRNA expression, DNA methylation and microRNA (miRNA) expression data. Built a patient network for each data type using Euclidean distance with a Gaussian kernel. Combined the networks using message-passing. Applied on METABRIC and compared with a couple of other methods.
  - Reid, Stephen, and Robert Tibshirani. "Sparse regression and marginal testing using cluster prototypes." [[http://biostatistics.oxfordjournals.org/content/early/2015/11/27/biostatistics.kxv049.short?rss=1|Biostatistics]] (2015). \\  Read this recent paper to follow the literature after LASSO.
  - Torkamani, Ali, and Nicholas J. Schork. "Identification of rare cancer driver mutations by network reconstruction." Genome [[http://genome.cshlp.org/content/19/9/1570.long|research]] 19.9 (2009): 1570-1578. \\  "Although there are many algorithms and strategies for reconstructing gene coexpression networks, including those that exploit a simple pairwise correlation matrix of gene-expression levels and clustering algorithms, the mutual information approach in the [[http://wiki.c2b2.columbia.edu/califanolab/index.php/Software/ARACNE|ARACNE]] algorithms has been shown to provide superior results in related contexts."
  - Wang, Jinlian, et al. "Pathway and network approaches for identification of cancer signature markers from omics data." //J [[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4278915/|Cancer]]//6.1 (2015): 54-65. \\ Surveyed integrating methods too.
  - Hill, Steven M., et al. "Inferring causal molecular networks: empirical assessment through a community-based effort." //[[http://www.nature.com/nmeth/journal/v13/n4/full/nmeth.3773.html?WT.ec_id=NMETH-201604&spMailingID=51038576&spUserID=MTIyMzczNjc4MDI2S0&spJobID=883886888&spReportId=ODgzODg2ODg4S0|Nature]] methods// (2016). \\ A DREAM challenge. Compared 2000 networks in 32 biological contexts.
  - Manning, Cerys Sian. Heterogeneity in melanoma and the microenvironment. [[http://discovery.ucl.ac.uk/1381937/1/Thesiswithcorrectionsaccept.pdf|Diss]]. UCL (University College London), 2013. \\  A PhD thesis with a good introduction to melanoma.
  - Shannan, Batool, et al. "Heterogeneity in Melanoma." Melanoma. Springer International Publishing, 2016. 1-15 [[:melanoma_book-2015.pdf?media=melanoma_book-2015.pdf|pdf]]]. \\  A chapter of a comprehensive recent book on melanoma.
  - Jiao, Yinming, Martin Widschwendter, and Andrew E. Teschendorff. "A systems-level integrative framework for genome-wide DNA methylation and gene expression data identifies differential gene expression modules under epigenetic control." Bioinformatics 30.16 (2014): 2360-2366. \\ [[https://academic.oup.com/bioinformatics/article-lookup/doi/10.1093/bioinformatics/btu316|FEM]] paper.
  - Comprehensive and Integrative Genomic Characterization of Hepatocellular Carcinoma, [[http://www.cell.com/cell/abstract/S0092-8674(17)30639-6?innerTabgraphical_S0092867417306396|Cell]], 2017 [{{:ally-copmprehensive_and_integrative_genomic_char_of_hcc-cell-2017.pdf|pdf}}  ]. \\  TCGA's HCC data and subtyping using DNA copy number, DNA methylation, mRNA expression, miRNA expression and RPPA (protein expression). Links to the MDACC dataset with 100 HCC samples.
  - Guillamot, Maria, Luisa Cimmino, and Iannis Aifantis. "The impact of DNA methylation in hematopoietic malignancies." [[http://www.cell.com/trends/cancer/pdf/S2405-8033(15)00089-8.pdf|Trends in cancer]] 2.2 (2016): 70-83. \\  Reviews and references DNA methylation studies and datasets on AML. E.g., [[http://www.sciencedirect.com/science/article/pii/S1535610809004206|Figueroa]] et al. used DNA methylation for classification of 344 AML cases. [[http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1002781|Akalin]] et al. related DNA methylation patterns with mutations in 5 AML cases. "The methylation status of specific genes can predict the future survival of AML patients, suggesting that DNA methylation is a biomarker for clinical outcome" see e.g., Figueroa et al, [[http://www.bloodjournal.org/content/bloodjournal/113/6/1315.full.pdf?sso-checked=true|Jiang]] 2009 (studied MDS to AML progression in 184 cases), and [[http://www.bloodjournal.org/content/115/3/636.long?sso-checked=true|Bullinger]] 2010 (analyzed 92 genomic regions in 182 patients).

\\ **Related software**

- Weighted Gene Co-expression Network Analysis ([[http://labs.genetics.ucla.edu/horvath/CoexpressionNetwork/|WGCNA]]) developed at UCLA. The page has links to some good introductory workshops.
  - [[http://www.bioconductor.org/packages/2.10/bioc/html/birta.html|birta]] (Bayesian Inference of Regulation of Transcriptional Activity) uses the regulatory networks of TFs and miRNAs together with mRNA and miRNA expression data to predict switches in regulatory activity between two conditions. A Bayesian network is used to model the regulatory structure.
  - [[http://www.bioconductor.org/packages/devel/bioc/html/GRENITS.html|GRENITS]], Regulatory Network Inference Using Time Series, is based on Dynamic Bayesian Networks.
  - [[http://www.cs.duke.edu/~amink/software/banjo/|BANJO]], a Java software for structure learning of static and dynamic Bayesian networks developed at Duke University [[:http:www.ploscompbiol.org_article_info:doi_10.1371_journal.pcbi.0020161#pcbi-0020161-g005|paper]] (DBN)]. No inference, BDe score, simulated annealing and greedy searches, handles thousands of variables, used for gene network inference. "In our experience the simulated annealer method [with RandomLocal] seems to produce the best results." Computes consensus graph, discretization, Forces or disallows edges by "network structure properties" which is useful for merging networks.
  - [[http://www.c2b2.columbia.edu/danapeerlab/html/biolearn.html|Biolearn]], applies probabilistic graphical models to biological data. Not maintained anymore.
  - [[http://cran.r-project.org/web/packages/deal/index.html|deal]]: an R package for Learning Bayesian Networks. Greedy search.
  - Software Packages for Graphical Models (2007), Murphy's [[http://www.cs.ubc.ca/~murphyk/Software/bnsoft.html|list]]. See our **refined [[:comparison_of_bayesian_network_learners|comparison]] of Bayesian network learners**.
  - [[http://cran.r-project.org/web/views/gR.html|gr]], gRaphical Models in R
  - [[http://icahn.mssm.edu/departments-and-institutes/genomics/about/software/rimbanet|RIMBANet]], reconstructs integrative molecular Bayesian networks, maybe used to study Alzheimer’s Disease in the Cell paper.
  - [[http://www.cs.waikato.ac.nz/ml/weka/|Weka]], a collection of machine learning tools including learning [[http://weka.sourceforge.net/manuals/weka.bn.pdf|BNs]]. Has R interface. Simulated annealing but no other MCMC (their [[http://www.sciencedirect.com/science/article/pii/S0096300309001362|relation]]). No inference instead recommends javabayes which accepts XML BIF format. Java classes are flexible enough to allow for implementing new learners.
  - [[http://www.bnlearn.com/|bnlearn]], an R package for learning BNs which [[http://www.bnlearn.com/bnrepository/|claims]] to learn a ~1000-nodes network (introductory [[https://urldefense.proofpoint.com/v2/url?u=http-3A__www.edii.uclm.es_-7EuseR-2D2013_Tutorials_scutari_tuta04.zip&d=AwMFaQ&c=OrYO-caJHQE1g_AJU3az1awi55It-bjDIQrtRiZ6WBk&r=Gu0CM1-T0ibkkQ0zAp7yDQ&m=Oz-QkJuAJrHgtZFVh4dIaSB2CM5XTcHSg-SPRlG-slU&s=4e3Php2tlmsXxWKP3r9OSHvBWRjIjbic0VWgTz1iGOs&e=|slides]]). A descriptive [[http://arxiv.org/pdf/0908.3817.pdf|comparison]] with other R packages. No MCMC. See Radhakrishnan et al. above.
  - [[http://www.cytoscape.org/what_is_cytoscape.html|Cytoscape]], visualizes molecular interaction networks and biological pathways.
  - **[[http://sign.hgc.jp/signbn/|SiGN-BN ]],**described [[http://ieeexplore.ieee.org/ielx5/8857/5730188/05551118.pdf?tp=&arnumber=5551118&isnumber=5730188|here]], compared with BANJO and more [[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3315333/|here]].
  - See the [[:comparison_of_bayesian_network_learners|table]] for comparison of methods for learning BNs related to our gene network project.

\\  \\ [[:drafts|Drafts]], [[:next_steps|Next steps]]

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