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hepatocellular_carcinoma [2020/12/08 18:18]
admin [Project 3:]
hepatocellular_carcinoma [2020/12/16 02:03] (current)
admin [Project 3:]
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 **Protemoe and the APEX1 interactome:​** Data-independent analysis Mass spectrometry (DIA-MS) done on 3 biological replicates of 2 HCC cell lines (SNU398 and Huh7) and an immortalized hepatocyte line (THLE2). Also, we have 1 biological replicate of a primary hepatocyte organoid derived from a patient (UTHSS-28T). **Protemoe and the APEX1 interactome:​** Data-independent analysis Mass spectrometry (DIA-MS) done on 3 biological replicates of 2 HCC cell lines (SNU398 and Huh7) and an immortalized hepatocyte line (THLE2). Also, we have 1 biological replicate of a primary hepatocyte organoid derived from a patient (UTHSS-28T).
  
-**Our goal** is to understand if the APE1 interactome is 1) different in HCC cell lines vs non tumor cells, 2) different between two HCC cell lines with {{:​apex1_analysis_of_dia-ms_dc_139-12-1-20.pptx|overexpressed}} ​ APEX1 (SNU398 vs Huh7), 3) and how it compares to that described in [[https://​www.nature.com/​articles/​s41598-019-56981-z|Ayyildiz 2020]]. Christi sent these data to Habil on 2020-12-02 in an email entitled: "​M2021-026 Scaffold DIA and Excel files"​.+**Our goal** is to understand if the APE1 interactome is 1) different in HCC cell lines vs non tumor cells, 2) different between two HCC cell lines with {{:​apex1_analysis_of_dia-ms_dc_139-12-1-20.pptx|overexpressed}} ​ APEX1 (SNU398 vs Huh7), 3) and how it compares to that described in [[https://​www.nature.com/​articles/​s41598-019-56981-z|Ayyildiz 2020]]. Christi sent these data to Habil on 2020-12-02 in an email entitled: "​M2021-026 ​{{:​hcc-in-vitro-proteome-scaffold-dia-2020-12-02.7z|Scaffold}}  ​DIA and Excel files"​.