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hepatocellular_carcinoma [2020/12/07 22:41] – [Project 3:] admin | hepatocellular_carcinoma [2020/12/16 00:03] (current) – [Project 3:] admin |
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==== Project 3: ==== | ==== Project 3: ==== |
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**Protemoe and the APEX1 interactome:** Data-independent analysis Mass spectrometry (DIA-MS) done on 3 HCC cell lines and an immortalized hepatocyte line, each with 3 biological replicates. Our goal is to understand if the APE1 interactome is 1) different in HCC cell lines vs non tumor cells, 2) different between HCC cell lines with overexpressed APEX1 (SNU398 vs Huh7), 3) and how it compares to that described in [[https://www.nature.com/articles/s41598-019-56981-z|Ayyildiz 2020]]. Christi sent these data to Habil on 2020-12-02 in an email entitled: "M2021-026 Scaffold DIA and Excel files". | **Protemoe and the APEX1 interactome:** Data-independent analysis Mass spectrometry (DIA-MS) done on 3 biological replicates of 2 HCC cell lines (SNU398 and Huh7) and an immortalized hepatocyte line (THLE2). Also, we have 1 biological replicate of a primary hepatocyte organoid derived from a patient (UTHSS-28T). |
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| **Our goal** is to understand if the APE1 interactome is 1) different in HCC cell lines vs non tumor cells, 2) different between two HCC cell lines with {{:apex1_analysis_of_dia-ms_dc_139-12-1-20.pptx|overexpressed}} APEX1 (SNU398 vs Huh7), 3) and how it compares to that described in [[https://www.nature.com/articles/s41598-019-56981-z|Ayyildiz 2020]]. Christi sent these data to Habil on 2020-12-02 in an email entitled: "M2021-026 {{:hcc-in-vitro-proteome-scaffold-dia-2020-12-02.7z|Scaffold}} DIA and Excel files". |
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