✎ rapamycin [oncinfo]

<h1>Rapamycin effects on aging-associated pathologies</h1>
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<h2>Objectives</h2>
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<a href="https://en.wikipedia.org/wiki/Sirolimus" class="urlextern" title="https://en.wikipedia.org/wiki/Sirolimus" rel="ugc nofollow">Rapamycin</a> is an inhibitor of the <a href="https://en.wikipedia.org/wiki/MTOR" class="urlextern" title="https://en.wikipedia.org/wiki/MTOR" rel="ugc nofollow">mTOR</a> pathway and it has been used in preventing rejection of solid organ allografts. In this multi-PI study on an older age healthy human cohort, we assess the usability and efficacy of Rapamycin in improving physical performance, cardiac and vascular health, and immune outcomes. This approach is motivated by the promising results that were observed in prior studies on mice models. Oncinfo contributes in this study through analysis of gene expression and DNA methylation profiles.
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<h2>Data</h2>
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<li class="level1"> DNA methylation data of <a href="/lib/exe/fetch.php?media=wiki:public:plan_discussed_with_habil_07_24_19.docx" class="media mediafile mf_docx" title="wiki:public:plan_discussed_with_habil_07_24_19.docx (17.2 KB)">29 subjects</a>  were measured in 2019 using Infinium <a href="https://support.illumina.com/downloads/infinium-methylationepic-v1-0-product-files.html" class="urlextern" title="https://support.illumina.com/downloads/infinium-methylationepic-v1-0-product-files.html" rel="ugc nofollow">MethylationEpic</a> V1 Array. PBMC samples were collected before and after Rapamycin (16 subjects) and placebo (13 subjects) treatment for 6-8 weeks.</li>
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<h2>Collaborators</h2>
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Dr. <a href="https://wp.uthscsa.edu/mimg/team-member/ellen-kraig-ph-d/" class="urlextern" title="https://wp.uthscsa.edu/mimg/team-member/ellen-kraig-ph-d/" rel="ugc nofollow">Ellen Kraig</a>, an immunologist, and Dr. <a href="https://www.uthscsa.edu/academics/biomedical-sciences/faculty/profile/0V71EU5IM/Kellogg,-Dean-L.-Jr." class="urlextern" title="https://www.uthscsa.edu/academics/biomedical-sciences/faculty/profile/0V71EU5IM/Kellogg,-Dean-L.-Jr." rel="ugc nofollow">Dean Kellogg</a>, a physiologist, both from the University of Texas Health Science Center at San Antonio.
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<h2>DNA methylation analysis</h2>
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We follow the Bioconductor <a href="https://www.bioconductor.org/packages/release/workflows/vignettes/methylationArrayAnalysis/inst/doc/methylationArrayAnalysis.html#differential-methylation-analysis" class="urlextern" title="https://www.bioconductor.org/packages/release/workflows/vignettes/methylationArrayAnalysis/inst/doc/methylationArrayAnalysis.html#differential-methylation-analysis" rel="ugc nofollow">workflow</a> to identify the probes and regions that are differentially methylation due to Rapamycin treatment.
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<h2>Related work</h2>
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<li class="level1"> Kraig, Ellen, et al. "A randomized control trial to establish the feasibility and safety of rapamycin treatment in an older human cohort: Immunological, physical performance, and cognitive effects." <a href="https://www.sciencedirect.com/science/article/pii/S0531556517309130" class="urlextern" title="https://www.sciencedirect.com/science/article/pii/S0531556517309130" rel="ugc nofollow">Experimental gerontology</a> 105 (2018): 53-69.</li>
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